Difference between revisions of "Part:BBa K2150016"
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T7 promoter and RBS and GFP, with double terminator. Though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase. | T7 promoter and RBS and GFP, with double terminator. Though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
We use this part to produce a high level of reporter protein GFP in our simulation system where T7 RNA polymerase is present. We observed the T7 promoter is leaky on high-copy plasmid pSB1C3. | We use this part to produce a high level of reporter protein GFP in our simulation system where T7 RNA polymerase is present. We observed the T7 promoter is leaky on high-copy plasmid pSB1C3. | ||
Revision as of 19:41, 19 October 2016
pT7 GFP
T7 promoter and RBS and GFP, with double terminator. Though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase.
Usage and Biology
We use this part to produce a high level of reporter protein GFP in our simulation system where T7 RNA polymerase is present. We observed the T7 promoter is leaky on high-copy plasmid pSB1C3.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 690