Difference between revisions of "Part:BBa K1896016"
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The truncated INP is still capable of catalysing the formation of ice crystals, but is no longer localised to the outer cell membrane [1].
 
The truncated INP is still capable of catalysing the formation of ice crystals, but is no longer localised to the outer cell membrane [1].
 
A monomeric GFP variant ([[Part:BBa_K1896001|mGFPuv2]]) has been fused to INP<sub>RC</sub> to act as a control in protein adhesion experiments performed by the UGent Belgium 2016 iGEM team.
 
A monomeric GFP variant ([[Part:BBa_K1896001|mGFPuv2]]) has been fused to INP<sub>RC</sub> to act as a control in protein adhesion experiments performed by the UGent Belgium 2016 iGEM team.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1896016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1896016 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 19:31, 19 October 2016


INP_RC-mGFPuv2 generator

This part contains the INPRC-mGFPuv2 fusion protein controlled by a weak to medium constitutive promoter and RBS.

Usage and Biology

INPRC is a truncated Ice Nucleating Protein from which the N-terminal domain has been removed. The truncated INP is still capable of catalysing the formation of ice crystals, but is no longer localised to the outer cell membrane [1]. A monomeric GFP variant (mGFPuv2) has been fused to INPRC to act as a control in protein adhesion experiments performed by the UGent Belgium 2016 iGEM team.

+++insert movie+++

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 203
    Illegal NgoMIV site found at 1715
    Illegal NgoMIV site found at 1859
    Illegal NgoMIV site found at 2123
    Illegal NgoMIV site found at 2267
    Illegal NgoMIV site found at 2315
    Illegal NgoMIV site found at 2435
    Illegal AgeI site found at 1379
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Green, R. L., Corotto, L. V., & Warren, G. J. (1988). Deletion mutagenesis of the ice nucleation gene from Pseudomonas syringae S203. Molecular and General Genetics MGG, 215(1), 165-172.