Difference between revisions of "Part:BBa K1896011"
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This is an improved version of [[Part:BBa_K584027|BBa_K584027]], which expresses an Ice Nucleating Protein, encoded by the [[Part:BBa_K1896002|''inaZ'']] gene of ''Pseudomonas syringae''. The original part was not genetically stable, probably due to toxicity caused by overexpression of the membrane bound INP. Replacing the promoter with a weaker variant proved to be effective in reducing toxicity, while still maintaining activity as biological ice nucleator. This activity is the result of a central repeat domain that catalyses the formation of ice crystals by binding molecules of water in a way that resembles the lattice structure of ice. [1]
 
This is an improved version of [[Part:BBa_K584027|BBa_K584027]], which expresses an Ice Nucleating Protein, encoded by the [[Part:BBa_K1896002|''inaZ'']] gene of ''Pseudomonas syringae''. The original part was not genetically stable, probably due to toxicity caused by overexpression of the membrane bound INP. Replacing the promoter with a weaker variant proved to be effective in reducing toxicity, while still maintaining activity as biological ice nucleator. This activity is the result of a central repeat domain that catalyses the formation of ice crystals by binding molecules of water in a way that resembles the lattice structure of ice. [1]
  
−
+++inaZ movie+++
+
[[File:BBa K1896011 inaZ.mp4|400px|thumb|centre|
 +
Addition of ''E. coli'' expressing mRFP to supercooled water has no effect,  addition of a culture containing this part instantly causes ice nucleation catalysed by INP.]]
  
  

Revision as of 19:31, 19 October 2016


inaZ (INP) generator

This is an improved version of BBa_K584027, which expresses an Ice Nucleating Protein, encoded by the inaZ gene of Pseudomonas syringae. The original part was not genetically stable, probably due to toxicity caused by overexpression of the membrane bound INP. Replacing the promoter with a weaker variant proved to be effective in reducing toxicity, while still maintaining activity as biological ice nucleator. This activity is the result of a central repeat domain that catalyses the formation of ice crystals by binding molecules of water in a way that resembles the lattice structure of ice. [1]

File:BBa K1896011 inaZ.mp4


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 707
    Illegal NgoMIV site found at 2219
    Illegal NgoMIV site found at 2363
    Illegal NgoMIV site found at 2627
    Illegal NgoMIV site found at 2771
    Illegal NgoMIV site found at 2819
    Illegal NgoMIV site found at 2939
    Illegal AgeI site found at 478
    Illegal AgeI site found at 1883
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 299


References

  1. Pandey, R., Usui, K., Livingstone, R. A., Fischer, S. A., Pfaendtner, J., Backus, E. H., ... & Scheel, J. F. (2016). Ice-nucleating bacteria control the order and dynamics of interfacial water. Science advances, 2(4), e1501630.