Difference between revisions of "Part:BBa K1896017"
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<partinfo>BBa_K1896017 short</partinfo> | <partinfo>BBa_K1896017 short</partinfo> | ||
− | + | This part contains the INP<sub>RC</sub>-streptavidin fusion protein controlled by a weak to medium constitutive promoter and RBS. | |
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | [[Part:BBa_K1896008|INP<sub>RC</sub>]] is a truncated Ice Nucleating Protein from which the N-terminal domain has been removed. | ||
+ | The truncated INP is still capable of catalysing the formation of ice crystals, but is no longer localised to the outer cell membrane [1]. | ||
+ | The biotin-binding protein [[Part:BBa_K1896004|Streptavidin]] has been fused to INP<sub>RC</sub> for use in protein adhesion experiments performed by the UGent Belgium 2016 iGEM team. | ||
+ | This part appears to be toxic and accrues mutations, possibly due to the tetrameric nature of Streptavidin. To remedy this, a version with a monomeric Streptavidin variant was constructed ([[Part:BBa_K1896018|BBa_K1896018]]). | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1896017 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1896017 SequenceAndFeatures</partinfo> | ||
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<partinfo>BBa_K1896017 parameters</partinfo> | <partinfo>BBa_K1896017 parameters</partinfo> | ||
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+ | ===References=== | ||
+ | # Green, R. L., Corotto, L. V., & Warren, G. J. (1988). Deletion mutagenesis of the ice nucleation gene from ''Pseudomonas syringae S203''. ''Molecular and General Genetics MGG'', 215(1), 165-172. |
Latest revision as of 19:18, 19 October 2016
INP_RC-streptavidin generator
This part contains the INPRC-streptavidin fusion protein controlled by a weak to medium constitutive promoter and RBS.
Usage and Biology
INPRC is a truncated Ice Nucleating Protein from which the N-terminal domain has been removed. The truncated INP is still capable of catalysing the formation of ice crystals, but is no longer localised to the outer cell membrane [1]. The biotin-binding protein Streptavidin has been fused to INPRC for use in protein adhesion experiments performed by the UGent Belgium 2016 iGEM team. This part appears to be toxic and accrues mutations, possibly due to the tetrameric nature of Streptavidin. To remedy this, a version with a monomeric Streptavidin variant was constructed (BBa_K1896018).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 203
Illegal NgoMIV site found at 1715
Illegal NgoMIV site found at 1859
Illegal NgoMIV site found at 2123
Illegal NgoMIV site found at 2267
Illegal NgoMIV site found at 2315
Illegal NgoMIV site found at 2435
Illegal AgeI site found at 1379
Illegal AgeI site found at 3254
Illegal AgeI site found at 3305 - 1000COMPATIBLE WITH RFC[1000]
References
- Green, R. L., Corotto, L. V., & Warren, G. J. (1988). Deletion mutagenesis of the ice nucleation gene from Pseudomonas syringae S203. Molecular and General Genetics MGG, 215(1), 165-172.