Difference between revisions of "Part:BBa K1896001"
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* '''A206K''': Monomerizes most GFP variants by disrupting a hydrophobic patch. [3] | * '''A206K''': Monomerizes most GFP variants by disrupting a hydrophobic patch. [3] | ||
− | [[File:BBa K1896001 fusionProteins.jpg| | + | [[File:BBa K1896001 fusionProteins.jpg|600px|thumb|centre|MGFPuv2 can be used in difficult fusion proteins, from left to right: non-fluorescent control, INP<sub>RC</sub>-mGFPuv2 (weak promoter), mGFPuv2-streptavidin, mGFPuv2-mSA2, mGFPuv2]] |
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 19:00, 19 October 2016
mGFPuv2
Variant of Green Fluorescent Protein (GFP) that contains the following mutations:
- F99S/M153T/V163A: GFPuv or cycle 3 GFP was optimised for excitation by UV light (360-400nm). [1]
- S208L: Increases the fluorescence intensity of GFPuv, resulting in GFPuv2. [2]
- A206K: Monomerizes most GFP variants by disrupting a hydrophobic patch. [3]
Usage and Biology
This combination of GFP mutations has not been reported in the literature. The UGent Belgium 2016 iGEM team used this part as a single protein and as a fusion partner with mSA2 and INPRC
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- von Stetten, D., Noirclerc-Savoye, M., Goedhart, J., Gadella, T. W., & Royant, A. (2012). Structure of a fluorescent protein from Aequorea victoria bearing the obligate-monomer mutation A206K. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 68(8), 878-882.
- Ito, Y., Suzuki, M., & Husimi, Y. (1999). A novel mutant of green fluorescent protein with enhanced sensitivity for microanalysis at 488 nm excitation. Biochemical and biophysical research communications, 264(2), 556-560.
- Crameri, A., Whitehorn, E. A., Tate, E., Stemmer, W. P., Crameri, A., Kitts, P. A., & Kitts, P. A. (1996). Improved green fluorescent protein by molecular evolution using. Nat. Biotechnol, 14(3), 315-319.