Difference between revisions of "Part:BBa I20249"

Latest revision as of 13:34, 16 August 2007

Device used to characterize Ribosome Binding Sites through measurement of GFP expression

Device is based on I2055 and contains a partial biobrick site (Spe1, Not1, Xba1) between R0040 and GFP. To insert a BB promoter for testing, digest the promoter Xba1 and Spe1 and the kit EcoR1 and Xba1. Use .5ul of each restriction enzyme during the ligation to ensure the formation of two mixed sites. This design provides the proper spacing, identical to actual construction conditions.

Note: Sequence features are wrong

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 76
Illegal SpeI site found at 62
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 62
Illegal NotI site found at 68
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 76
Illegal SpeI site found at 62
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 76
Illegal SpeI site found at 62
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 724

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_I20249 short</partinfo>
 Device is based on I2055 and contains a partial biobrick site (Spe1, Not1, Xba1) between R0040 and GFP. To insert a BB promoter for testing, digest the promoter Xba1 and Spe1 and the kit EcoR1 and Xba1. Use .5ul of each restriction enzyme during the ligation to ensure the formation of two mixed sites. This design provides the proper spacing, identical to actual construction conditions.
+Note: Sequence features are wrong
 <!-- Add more about the biology of this part here