Difference between revisions of "Part:BBa I20245"
 
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Device is based on E0240 and contains a partial biobrick site (EcoR1, Not1, Xba1) upstream of the RBS. To insert a BB promoter for testing, digest the promoter EcoR1 and Spe1, and the result will form a mixed site. This design best simulates spacing in actual construction however, parts cannot be removed after testing.
 
Device is based on E0240 and contains a partial biobrick site (EcoR1, Not1, Xba1) upstream of the RBS. To insert a BB promoter for testing, digest the promoter EcoR1 and Spe1, and the result will form a mixed site. This design best simulates spacing in actual construction however, parts cannot be removed after testing.
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Note: Sequence Features are wrong
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:25, 16 August 2007

A device used to characterize promoters throught measurement of GFP expression

Device is based on E0240 and contains a partial biobrick site (EcoR1, Not1, Xba1) upstream of the RBS. To insert a BB promoter for testing, digest the promoter EcoR1 and Spe1, and the result will form a mixed site. This design best simulates spacing in actual construction however, parts cannot be removed after testing.

Note: Sequence Features are wrong

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 15
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 15
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 684