Difference between revisions of "Part:BBa K1985010"
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<partinfo>BBa_K1985010 short</partinfo>
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1985010 SequenceAndFeatures</partinfo>
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This part is an improved version of a previously designed BioBrick (Part:BBa_K1739002), which was designed by the Kent 2015 iGEM team. This part contains three segments, the CsgA signal sequence, Sup35NM and a prion forming domain. Our improved BioBrick aims to optimize the aggregation process of amyloid fibrils with the addition of a short and specific  prion forming domain of 61 aminoacid residues. This was inserted into the pSB1C3 backbone.
 
  
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===Usage and Biology===
 
===Usage and Biology===
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This part is an improved version of a previously designed BioBrick (Part:BBa_K1739002) from the Kent 2015 iGEM team. This part contains two segments, the CsgA signal sequence and only the first 61 aminoacids of the prion domain Sup35. Our improved BioBrick aims to optimize the self-assembly process of amyloid fibrils with the addition of these residues as they have been considered to be a suitable building block for the assembly of functional nanostructures.
  
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===Validation===
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The plasmid was analysed through a diagnostic double restriction cut, using the enzymes NdeI and SspI. This was followed by agarose gel electrophoresis. The enzymes cleave the insert and a part of the plasmid at 1618bp, with the remainder plasmid being 790 bp. The sizes of the two fragments were compared with the size of the Invitrogen 1kB DNA marker and was found that our fragments were the correct
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[[File:PSB1C3+61.jpeg||400px|thumb|centre|Figure 1.1% agarose gel of the restriction digest of BBa_K1985010 in pSB1C3 plasmid backbone with NdeI and SspI]]
  
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[[File:PSB1C3+61.jpeg||400px|thumb|centre|Figure 1. Writing about pic goes here]]
 
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K1985010 SequenceAndFeatures</partinfo>
 
  
  

Revision as of 18:07, 19 October 2016


Sequence coding for amyloid Sup35 residues 1-61 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This part is an improved version of a previously designed BioBrick (Part:BBa_K1739002) from the Kent 2015 iGEM team. This part contains two segments, the CsgA signal sequence and only the first 61 aminoacids of the prion domain Sup35. Our improved BioBrick aims to optimize the self-assembly process of amyloid fibrils with the addition of these residues as they have been considered to be a suitable building block for the assembly of functional nanostructures.

Validation

The plasmid was analysed through a diagnostic double restriction cut, using the enzymes NdeI and SspI. This was followed by agarose gel electrophoresis. The enzymes cleave the insert and a part of the plasmid at 1618bp, with the remainder plasmid being 790 bp. The sizes of the two fragments were compared with the size of the Invitrogen 1kB DNA marker and was found that our fragments were the correct

Figure 1.1% agarose gel of the restriction digest of BBa_K1985010 in pSB1C3 plasmid backbone with NdeI and SspI