Difference between revisions of "Part:BBa K1886006:Design"

(→‎References)
(→‎References)
 
Line 20: Line 20:
 
Olson E J, Hartsough L A, Landry B P, et al. Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals[J]. Nature methods, 2014, 11(4): 449-455.
 
Olson E J, Hartsough L A, Landry B P, et al. Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals[J]. Nature methods, 2014, 11(4): 449-455.
 
MLA
 
MLA
 +
 +
Synthetic biology: Engineering Escherichia coli to see light[J]. Nature 438, 441-442 (24 November 2005) | doi:10.1038/nature04405; Published online 23 November 2005

Latest revision as of 18:02, 19 October 2016


broken Ptet-cph8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2332
    Illegal XhoI site found at 439
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There was a SpeI site in the sequence encoding protein cph8, so we designed a silent mutation.



Source

From E coli genome, obtained from iGEM team HZAU's lab. This plasmid is bought from Addgene Company.

References

Olson E J, Hartsough L A, Landry B P, et al. Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals[J]. Nature methods, 2014, 11(4): 449-455. MLA

Synthetic biology: Engineering Escherichia coli to see light[J]. Nature 438, 441-442 (24 November 2005) | doi:10.1038/nature04405; Published online 23 November 2005