Difference between revisions of "Part:BBa K1959000"
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<partinfo>BBa_K1959000 short</partinfo>
 
<partinfo>BBa_K1959000 short</partinfo>
  
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The BBa_K1959000 harbors a coding sequence of phytoene synthase (PSY) derived from maize (''Zea mays''). Codon-optimization has been made for rice (''Oryza sativa''). PSY catalyzes the conversion of geranylgenranyl diphosphate to phytoene, which is a key enzyme for carotenoid biosynthesis.
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The BBa_K1959000 harbors a coding sequence of phytoene synthase (PSY) derived from maize (''Zea mays''). Codon-optimization has been made for rice (''Oryza sativa''). PSY catalyzes the conversion of geranylgenranyl diphosphate (GGDP) to phytoene, which is a key enzyme for carotenoid biosynthesis.
  
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===Usage and Biology===
 
===Usage and Biology===
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　Phytoene synthase (PSY, EC 2.5.1.32) is an enzyme involved and responded for the first synthetic reaction in β-carotene biosynthetic pathway. In this reaction, two GGDP are converted to one molecule phytoene and two diphosphates (Figure. 1). ''PSY'' genes had been applied to the nutrition-improved crops such as Golden Rice. ''PSY'' gene of maize (''ZmPSY'') was introduced into rice genome to achieve the synthesis of β-carotene. In our project, the ''ZmPSY'' was used to reconstruct the pathway of astaxanthin biosynthesis. For maize is a monocots similar to rice, thus the ''ZmPSY'' was not fused with transit peptide for plastid sorting.
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===Transcriptional activity of ''PSY''===
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　Semi-quantitative RT-PCR was performed to detect the expression level of ''PSY'' involved in astaxanthin biosynthesis, total RNA of transgenic rice seeds were extracted and cDNA was synthesized from 1μg DNase-treated RNA.
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Revision as of 17:15, 19 October 2016


Modification of Phytoene synthase (PSY)

The BBa_K1959000 harbors a coding sequence of phytoene synthase (PSY) derived from maize (Zea mays). Codon-optimization has been made for rice (Oryza sativa). PSY catalyzes the conversion of geranylgenranyl diphosphate (GGDP) to phytoene, which is a key enzyme for carotenoid biosynthesis.

Usage and Biology

　Phytoene synthase (PSY, EC 2.5.1.32) is an enzyme involved and responded for the first synthetic reaction in β-carotene biosynthetic pathway. In this reaction, two GGDP are converted to one molecule phytoene and two diphosphates (Figure. 1). PSY genes had been applied to the nutrition-improved crops such as Golden Rice. PSY gene of maize (ZmPSY) was introduced into rice genome to achieve the synthesis of β-carotene. In our project, the ZmPSY was used to reconstruct the pathway of astaxanthin biosynthesis. For maize is a monocots similar to rice, thus the ZmPSY was not fused with transit peptide for plastid sorting.

Transcriptional activity of PSY

　Semi-quantitative RT-PCR was performed to detect the expression level of PSY involved in astaxanthin biosynthesis, total RNA of transgenic rice seeds were extracted and cDNA was synthesized from 1μg DNase-treated RNA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1045
    Illegal NheI site found at 1164
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 105
    Illegal AgeI site found at 956
  • 1000
    COMPATIBLE WITH RFC[1000]