Difference between revisions of "Part:BBa K2170050"

Revision as of 17:01, 19 October 2016

Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin

This BioBrick contains a biotin binding receptor with enhanced monomeric Avidin, which is presented on the cellsurface of procaryotic cell to bind biotin and with this, imparts the polymerization of our cells. Because of the OmpA the receptor is secreted via the SEC pathway into the outer membrane. The EspP-Autotransporter, which is on the C-terminal side of the fusion protein, pushes the eMA through the outer membrane and presents it on the cell surface where it is ready to bind biotinylated protein linkers. For localisation analysis an A3C5 Tag was added to the construct. The expression is controlled controlled by a TetR-TetO synstem and can be induced by addition of Tetracycline.

TetR_TetO_OmpA_eMA_A3C5_EspP_double terminator

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2448
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 889

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2170050 short</partinfo>
-Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin (TetR_TetO_OmpA_eMA_A3C5_EspP_double terminator)
+This BioBrick contains a biotin binding receptor with enhanced monomeric Avidin, which is presented on the cellsurface of procaryotic cell to bind biotin and with this, imparts the polymerization of our cells. Because of the OmpA the receptor is secreted via the SEC pathway into the outer membrane. The EspP-Autotransporter, which is on the C-terminal side of the fusion protein, pushes the eMA through the outer membrane and presents it on the cell surface where it is ready to bind biotinylated protein linkers. For localisation analysis an A3C5 Tag was added to the construct. The expression is controlled controlled by a TetR-TetO synstem and can be induced by addition of Tetracycline.
+TetR_TetO_OmpA_eMA_A3C5_EspP_double terminator