Difference between revisions of "Part:BBa K1886016"

Revision as of 16:48, 19 October 2016

AND GATE-input_ara+IPTG

This is the input part of AND GATE. After being induced by IPTG, plac promoter activates the expression of T7 RNA polymerase which contains two amber mutations (T7 ptag) . After being induced by L-arabinose, pbad promoter activates the expression of a special tRNA (supD), which is used to identify amber mutation. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase. Therefore, only when the two inputs exist together, T7 RNA polymerase can be expressed, and then T7 promoter can be activated to express the downstream gene.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 171
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 174
Illegal BsaI.rc site found at 4155

Revision as of 12:45, 7 October 2016 (view source) Registry (Talk \| contribs)		Revision as of 16:48, 19 October 2016 (view source) Lejian (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K1886016 short</partinfo>		<partinfo>BBa_K1886016 short</partinfo>

−	This is the input part of AND GATE. After being induced by ~~L-arabinose~~, ~~pbad~~ promoter activates the expression of T7 RNA polymerase which contains two amber mutations (T7 ptag) . After being induced by ~~IPTG~~, ~~plac~~ promoter activates the expression of a special tRNA (supD), which is used to identify amber mutation. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase. Therefore, only when the two inputs exist together, T7 RNA polymerase can be expressed, and then T7 promoter can be activated to express the downstream gene.	+	This is the input part of AND GATE. After being induced by IPTG, plac promoter activates the expression of T7 RNA polymerase which contains two amber mutations (T7 ptag) . After being induced by L-arabinose, pbad promoter activates the expression of a special tRNA (supD), which is used to identify amber mutation. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase. Therefore, only when the two inputs exist together, T7 RNA polymerase can be expressed, and then T7 promoter can be activated to express the downstream gene.