Difference between revisions of "Part:BBa I20244:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_I20244 short</partinfo>
 
<partinfo>BBa_I20244 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Does not contain a full BB site upstream of the RBS to best simulate spacing in actual Promoter/RBS construction, which forms an Xba1/Spe1 mixed site.
  
  

Latest revision as of 15:09, 15 August 2007

A device used to characterize promoters throught measurement of GFP expression


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal prefix found in sequence at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 15
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 15
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 684


Design Notes

Does not contain a full BB site upstream of the RBS to best simulate spacing in actual Promoter/RBS construction, which forms an Xba1/Spe1 mixed site.


Source

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References