Difference between revisions of "Part:BBa K2170052:Design"

(→‎References)
 
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'''Related BioBrick:'''
 
'''Related BioBrick:'''
−
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
+
*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170050 BBa_K2170050: Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin]<br>
−
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
+
*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170051 BBa_K2170051: Secretory prokaryotic biotin presenting receptor with biotin acceptor peptide]
  
 
'''Cloning details:'''<br>
 
'''Cloning details:'''<br>
−
<!--*Designed in RFC10/RFC23/RFC25-->
+
*Designed in RFC10
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
−
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
+
 
  
 
'''Quality control measures:'''<br>
 
'''Quality control measures:'''<br>
−
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
+
<!--*Test digestion using EcoRI & PstI
−
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
+
<!--*Sequencing using primer VF2 & VR
 
<!--*Part was partly sequenced/Part was totally sequenced-->
 
<!--*Part was partly sequenced/Part was totally sequenced-->
  
 
'''Backbone:'''<br>
 
'''Backbone:'''<br>
−
<!--*Backbone name: pSB1C3'/?backbone_name?-->
+
*Backbone name: pSB1C3
−
<!--*Resistance: Amp/Cp/Kan/-->
+
*Resistance: Cp
−
<!--*Copynumber: low/medium/high-->
+
*Copynumber: high
  
 
'''Protein coding:'''<br>
 
'''Protein coding:'''<br>
−
<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
+
*Protein: Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin [Nucleotide 1030 to 2445]-->
−
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
+
*Tag: internal A3C5 Tag
−
<!--*The protein encoded is posttranslationally modified by ???.-->
+
−
<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
+
  
 
'''Enzymatic activity:'''
 
'''Enzymatic activity:'''
−
<!--none/EC-number ?.?.?.?-->
+
*none
  
 
'''Cytotoxicity:'''<br>
 
'''Cytotoxicity:'''<br>
−
<!--none/not known/cytotoxic for ''organism name''-->
+
*none
  
 
'''Safety notes:'''<br>
 
'''Safety notes:'''<br>
−
<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
+
Known and anticipated sefety issues: none
−
<!--Known and anticipated security issues: none/other.-->
+
Known and anticipated security issues: none
  
−
'''Intellectual property:'''
 
−
<!--Information on patent situation.-->
 
−
<!--Intellectual property claims made by the authors.-->
 
  
−
'''Corresponding part author/authors:'''
 
−
<!--https://igem.org/User_Information.cgi?user_id=????/email-->
 
  
 
===Source===
 
===Source===
  
 
'''Source:'''<br>
 
'''Source:'''<br>
−
<!--*Commercial system: plasmid name, system name, company name-->
+
 
−
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
+
*Preexisting BioBrick BBa_I739001; BBa_B0015
−
<!--*Preexisting BioBrick ?Bba_number?-->
+
*Parts Synthesized by IDT
−
<!--*cDNA Clone: ?clone_name?, ?company_name?-->
+
*Professor Dr. Arne Skerra (Chair biological chemistry of TUM)
−
<!--*Synthesized by ?company_name?.-->
+
  
 
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
Line 72: Line 64:
 
Genesequence derived from ''Escherichia coli''
 
Genesequence derived from ''Escherichia coli''
 
<!--*Codonoptimized for ''?organism_name?''-->
 
<!--*Codonoptimized for ''?organism_name?''-->
−
<!--*Designed for the following Chassis: ''?organism-name?''-->
+
*Designed for the following Chassis: procaryotic cells
 
<!--*Statement about functionality in other chassis.-->
 
<!--*Statement about functionality in other chassis.-->
  

Latest revision as of 16:06, 19 October 2016


Secretory prokaryotic biotin binding receptor with single chain avidin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3735
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 889


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Cloning details:

  • Designed in RFC10


Quality control measures:

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • Protein: Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin [Nucleotide 1030 to 2445]-->
  • Tag: internal A3C5 Tag

Enzymatic activity:

  • none

Cytotoxicity:

  • none

Safety notes:
Known and anticipated sefety issues: none Known and anticipated security issues: none


Source

Source:

  • Preexisting BioBrick BBa_I739001; BBa_B0015
  • Parts Synthesized by IDT
  • Professor Dr. Arne Skerra (Chair biological chemistry of TUM)


Organism:
Genesequence derived from Escherichia coli

  • Designed for the following Chassis: procaryotic cells


References

Literature references:
1. [http://jcb.rupress.org/content/108/2/229.short Kozak, M. (1989). The scanning model for translation: an update. The Journal of cell biology, 108(2), 229-241.]
2. [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.000439 Kredel, S., Oswald, F., Nienhaus, K., Deuschle, K., Röcker, C., Wolff, M., ... & Wiedenmann, J. (2009). mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures. PloS one, 4(2), e4391.]
3. Bajar, B. T., Wang, E. S., Lam, A. J., Kim, B. B., Jacobs, C. L., Howe, E. S., ... & Chu, J. (2016). Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Scientific reports, 6.
4. [http://www.nature.com/nprot/journal/v2/n6/abs/nprot.2007.209.html Schmidt, T. G., & Skerra, A. (2007). The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nature protocols, 2(6), 1528-1535.]

Database references: