Difference between revisions of "Part:BBa K2036007"
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<partinfo>BBa_K2036007 parameters</partinfo> | <partinfo>BBa_K2036007 parameters</partinfo> | ||
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| + | <h2>Protein&promoter</h2> | ||
| + | <p>--CII and pRE</p> | ||
| + | <br> | ||
| + | <p> | ||
| + | CII (BBa_K2036000) functions as a transcriptional activator to direct promoter RE, so we constructed CII-TT-pRE-RBS-GFP-LVAssrAtag as test group and pRE-RBS-GFPLVAssrAtag as CK to see if CII efficiently activate pRE. | ||
| + | </p> | ||
| + | <br> | ||
| + | [[File:T--HUST-China--CII-pRE_plate.png|800px|thumb|center|Fig1: According to the Flourescence measurement curve above, we can see clearly that GFP level increased over time and it showed significant difference from CK.]] | ||
| + | <br> | ||
| + | [[File:T--HUST-China--Experiments-CII-pRE_Flou-detec.png|800px|thumb|center|Fig2: We also did Fluorescence microscope detection after 30, 120 and 240 minutes induction. According to the figture below, we can tell qualitively that pRE leakage are at relative low level and CII can efficiently activate the promoter.]] | ||
Revision as of 15:56, 19 October 2016
RBS-CII-RBS-CII, tandem expression
CII is a transcriptional activator of pRE, but it is normally degrade by Ftsh, and in this case,tandom expression maybe useful to activate pRE.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Protein&promoter
--CII and pRE
CII (BBa_K2036000) functions as a transcriptional activator to direct promoter RE, so we constructed CII-TT-pRE-RBS-GFP-LVAssrAtag as test group and pRE-RBS-GFPLVAssrAtag as CK to see if CII efficiently activate pRE.
