Difference between revisions of "Part:BBa K2170050:Design"
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'''Source:'''<br>
 
'''Source:'''<br>
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<!--*Commercial system: plasmid name, system name, company name-->
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<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
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*Preexisting BioBrick BBa_I739001; BBa_B0015
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<!--*Preexisting BioBrick ?Bba_number?-->
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*Parts Synthesized by IDT
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*Professor Dr. Arne Skerra (Chair biological chemistry of TUM)
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<!--*Synthesized by ?company_name?.-->
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<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
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Genesequence derived from ''Escherichia coli''
 
Genesequence derived from ''Escherichia coli''
 
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<!--*Codonoptimized for ''?organism_name?''-->
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<!--*Designed for the following Chassis: ''?organism-name?''-->
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*Designed for the following Chassis: procaryotic cells
 
<!--*Statement about functionality in other chassis.-->
 
<!--*Statement about functionality in other chassis.-->
  

Revision as of 15:51, 19 October 2016


Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 889


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Cloning details:

  • Designed in RFC10


Quality control measures:

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • Protein: Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin [Nucleotide 1030 to 2445]-->
  • Tag: internal A3C5 Tag

Enzymatic activity:

  • none

Cytotoxicity:

  • none

Safety notes:
Known and anticipated sefety issues: none Known and anticipated security issues: none


Source

Source:

  • Preexisting BioBrick BBa_I739001; BBa_B0015
  • Parts Synthesized by IDT
  • Professor Dr. Arne Skerra (Chair biological chemistry of TUM)


Organism:
Genesequence derived from Escherichia coli

  • Designed for the following Chassis: procaryotic cells


References

Literature references:

Database references: