Difference between revisions of "Part:BBa K2170050:Design"
Line 16: Line 16:
  
 
'''Related BioBrick:'''
 
'''Related BioBrick:'''
−
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
+
*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170052 BBa_K2170052: Secretory prokaryotic biotin binding receptor with single chain avidin]<br>
−
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
+
*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170051 BBa_K2170051: Secretory prokaryotic biotin presenting receptor with biotin acceptor peptide]
  
 
'''Cloning details:'''<br>
 
'''Cloning details:'''<br>
−
<!--*Designed in RFC10/RFC23/RFC25-->
+
*Designed in RFC10
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
−
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
+
 
  
 
'''Quality control measures:'''<br>
 
'''Quality control measures:'''<br>
−
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
+
<!--*Test digestion using EcoRI & PstI
−
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
+
<!--*Sequencing using primer VF2 & VR
 
<!--*Part was partly sequenced/Part was totally sequenced-->
 
<!--*Part was partly sequenced/Part was totally sequenced-->
  
 
'''Backbone:'''<br>
 
'''Backbone:'''<br>
−
<!--*Backbone name: pSB1C3'/?backbone_name?-->
+
*Backbone name: pSB1C3
−
<!--*Resistance: Amp/Cp/Kan/-->
+
*Resistance: Cp
−
<!--*Copynumber: low/medium/high-->
+
*Copynumber: high
  
 
'''Protein coding:'''<br>
 
'''Protein coding:'''<br>
−
<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
+
*Protein: Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin [Nucleotide 1030 to 2445]-->
−
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
+
*Tag: internal A3C5 Tag
−
<!--*The protein encoded is posttranslationally modified by ???.-->
+
−
<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
+
  
 
'''Enzymatic activity:'''
 
'''Enzymatic activity:'''
−
<!--none/EC-number ?.?.?.?-->
+
*none
  
 
'''Cytotoxicity:'''<br>
 
'''Cytotoxicity:'''<br>
−
<!--none/not known/cytotoxic for ''organism name''-->
+
*none
  
 
'''Safety notes:'''<br>
 
'''Safety notes:'''<br>
−
<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
+
Known and anticipated sefety issues: none
−
<!--Known and anticipated security issues: none/other.-->
+
Known and anticipated security issues: none
  
−
'''Intellectual property:'''
 
−
<!--Information on patent situation.-->
 
−
<!--Intellectual property claims made by the authors.-->
 
  
−
'''Corresponding part author/authors:'''
 
−
<!--https://igem.org/User_Information.cgi?user_id=????/email-->
 
  
 
===Source===
 
===Source===

Revision as of 15:43, 19 October 2016


Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 889


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Cloning details:

  • Designed in RFC10


Quality control measures:

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • Protein: Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin [Nucleotide 1030 to 2445]-->
  • Tag: internal A3C5 Tag

Enzymatic activity:

  • none

Cytotoxicity:

  • none

Safety notes:
Known and anticipated sefety issues: none Known and anticipated security issues: none


Source

Source:


Organism:
Genesequence derived from Escherichia coli


References

Literature references:

Database references: