Difference between revisions of "Part:BBa K2100037:Experience"
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===Applications of BBa_K2100037=== | ===Applications of BBa_K2100037=== | ||
| + | We characterized the repressor TAL14 by creating a plasmid containing TAL14 under the control of a doxycycline inducible promoter, TRE. This allowed for us to modulate the repressor expression by varying the concentrations of doxycycline added. We also created a repressible promoter controlling the expression of a fluorescent protein. This plasmid allowed for us to measure the repression occurring. | ||
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| + | https://static.igem.org/mediawiki/2016/a/ab/MIT_repressor_expt.png | ||
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| + | In order for us to analyze the level of repression, we need to see sustained presence. Upon increasing the concentration of doxycycline, we were unable to see that sustained presence of the repressors. From the results of testing TAL14 below, it can be seen that although there is activation early on, the production of repressor falls at 500 nM. This could possible be due to errors in doxycycline dilutions at 500 nM and above. | ||
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| + | [image here] | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K2100037 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K2100037 StartReviews</partinfo> | ||
Latest revision as of 15:38, 19 October 2016
This experience page is provided so that any user may enter their experience using this part.
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Applications of BBa_K2100037
We characterized the repressor TAL14 by creating a plasmid containing TAL14 under the control of a doxycycline inducible promoter, TRE. This allowed for us to modulate the repressor expression by varying the concentrations of doxycycline added. We also created a repressible promoter controlling the expression of a fluorescent protein. This plasmid allowed for us to measure the repression occurring.
In order for us to analyze the level of repression, we need to see sustained presence. Upon increasing the concentration of doxycycline, we were unable to see that sustained presence of the repressors. From the results of testing TAL14 below, it can be seen that although there is activation early on, the production of repressor falls at 500 nM. This could possible be due to errors in doxycycline dilutions at 500 nM and above.
[image here]
User Reviews
UNIQ6daceae14f1289b4-partinfo-00000000-QINU UNIQ6daceae14f1289b4-partinfo-00000001-QINU