Difference between revisions of "Part:BBa K2100018:Experience"
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===Applications of BBa_K2100018=== | ===Applications of BBa_K2100018=== | ||
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| + | We characterized the repressor BM3R1 by creating a plasmid TRE:BM3R1, containing BM3R1 under the control of a doxycycline inducible promoter, TRE. This allowed for us to modulate the repressor expression by varying the concentrations of doxycycline added. We also created a repressible promoter controlling the expression of a fluorescent protein. This plasmid allowed for us to measure the repression occurring. | ||
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| + | https://static.igem.org/mediawiki/2016/a/ab/MIT_repressor_expt.png | ||
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| + | In order for us to analyze the level of repression, we need to see sustained presence. Upon increasing the concentration of doxycycline, we were unable to see that sustained presence of the repressors. From the results of testing BM3R1 below, it can be seen that although there is activation early on, the production of repressor falls at 500 nM. This could possible be due to errors in doxycycline dilutions at 500 nM and above. | ||
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| + | https://static.igem.org/mediawiki/2016/e/e0/MIT_repressor_results.png | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 15:26, 19 October 2016
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Applications of BBa_K2100018
We characterized the repressor BM3R1 by creating a plasmid TRE:BM3R1, containing BM3R1 under the control of a doxycycline inducible promoter, TRE. This allowed for us to modulate the repressor expression by varying the concentrations of doxycycline added. We also created a repressible promoter controlling the expression of a fluorescent protein. This plasmid allowed for us to measure the repression occurring.
In order for us to analyze the level of repression, we need to see sustained presence. Upon increasing the concentration of doxycycline, we were unable to see that sustained presence of the repressors. From the results of testing BM3R1 below, it can be seen that although there is activation early on, the production of repressor falls at 500 nM. This could possible be due to errors in doxycycline dilutions at 500 nM and above.
User Reviews
UNIQ1bf80323240ba37b-partinfo-00000000-QINU UNIQ1bf80323240ba37b-partinfo-00000001-QINU