Difference between revisions of "Part:BBa K2100036:Experience"
 
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https://static.igem.org/mediawiki/2016/a/ab/MIT_repressor_expt.png
 
https://static.igem.org/mediawiki/2016/a/ab/MIT_repressor_expt.png
  
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In order for us to analyze the level of repression, we need to see sustained presence of the repressor. Upon increasing the concentration of doxycycline, we were unable to see that sustained presence of the repressors. From the results of testing BM3R1 below, it can be seen that although there is activation early on, the production of repressor falls at 500 nM. This could possible be due to errors in doxycycline dilutions at 500 nM and above.  
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In order for us to analyze the level of repression, we need to see sustained presence. Upon increasing the concentration of doxycycline, we were unable to see that sustained presence of the repressors. From the results of testing BM3R1 below, it can be seen that although there is activation early on, the production of repressor falls at 500 nM. This could possible be due to errors in doxycycline dilutions at 500 nM and above.  
  
 
https://static.igem.org/mediawiki/2016/e/e0/MIT_repressor_results.png
 
https://static.igem.org/mediawiki/2016/e/e0/MIT_repressor_results.png

Latest revision as of 15:24, 19 October 2016


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Please enter how you used this part and how it worked out.

Applications of BBa_K2100036

We characterized the repressor BM3R1 by creating a plasmid containing BM3R1 under the control of a doxycycline inducible promoter, TRE. This allowed for us to modulate the repressor expression by varying the concentrations of doxycycline added. We also created a repressible promoter controlling the expression of a fluorescent protein. This plasmid allowed for us to measure the repression occurring.


MIT_repressor_expt.png

In order for us to analyze the level of repression, we need to see sustained presence. Upon increasing the concentration of doxycycline, we were unable to see that sustained presence of the repressors. From the results of testing BM3R1 below, it can be seen that although there is activation early on, the production of repressor falls at 500 nM. This could possible be due to errors in doxycycline dilutions at 500 nM and above.

MIT_repressor_results.png


User Reviews

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