Difference between revisions of "Part:BBa K1941008"
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sgRNA that targets the TDH3 promoter. | sgRNA that targets the TDH3 promoter. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | The 20 bp of the TDH3 promoter targeted by this gRNA are located near the transcription start site. It can therefore perform CRISPRi when complexed with dCas9. | ||
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Latest revision as of 15:07, 19 October 2016
sgRNA c6_TDH3 (3)
sgRNA that targets the TDH3 promoter.
Usage and Biology
The 20 bp of the TDH3 promoter targeted by this gRNA are located near the transcription start site. It can therefore perform CRISPRi when complexed with dCas9.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 145
Illegal NgoMIV site found at 174 - 1000COMPATIBLE WITH RFC[1000]