Difference between revisions of "Part:BBa K1896015"

Revision as of 15:03, 19 October 2016

mGFPuv2-mSA2 generator

This part constitutively expresses a fusion protein comprising monomeric variants of GFPuv (mGFPuv2) and Streptavidin (mSA2). When the crude lysate of a culture of E. coli that contains this part is spread over solid biotinylated poly lactic acid (PLA), the strong affinity of mSA2 to biotin causes the fusion protein to adhere to the PLA. The biotin-mSA2 interaction is strong enough to resist multiple washes with saline.

PLA is a material often used for 3D printing and biotin can be incorporated either in the raw filament itself, or coated on the printed structure [http://2016.igem.org/Team:UGent_Belgium/Filament|http://2016.igem.org/Team:UGent_Belgium/Filament]. This part was created to demonstrate that mSA2 can be used to attach proteins to 3D printed structures. The combination of Synthetic Biology and 3D printing could greatly broaden the applicability of many genetic parts.

+++ Photo of attachment to biotin-PLA coated glass slides+++

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 872
Illegal AgeI site found at 932
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1896015 short</partinfo>
-This part constitutively expresses a fusion protein comprising monomeric variants of GFPuv ([[Part:BBa_K1896001|mGFPuv2]]) and Streptavidin ([[Part:BBa_K1896000|mSA2]]). When the crude lysate of a culture of ''E. coli'' that contains this part is spread over solid biotinylated poly lactic acid (PLA), the strong affinity of mSA2 causes the fusion protein to adhere to the PLA, resisting washing with saline.
+This part constitutively expresses a fusion protein comprising monomeric variants of GFPuv ([[Part:BBa_K1896001|mGFPuv2]]) and Streptavidin ([[Part:BBa_K1896000|mSA2]]). When the crude lysate of a culture of ''E. coli'' that contains this part is spread over solid biotinylated poly lactic acid (PLA), the strong affinity of mSA2 to biotin causes the fusion protein to adhere to the PLA. The biotin-mSA2 interaction is strong enough to resist multiple washes with saline.
+PLA is a material often used for 3D printing and biotin can be incorporated either in the raw filament itself, or coated on the printed structure [http://2016.igem.org/Team:UGent_Belgium/Filament|http://2016.igem.org/Team:UGent_Belgium/Filament]. This part was created to demonstrate that [[Part:BBa_K1896000|mSA2]] can be used to attach proteins to 3D printed structures. The combination of Synthetic Biology and 3D printing could greatly broaden the applicability of many genetic parts.
 +++ Photo of attachment to biotin-PLA coated glass slides+++