Difference between revisions of "Part:BBa K1919101"
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| − | + | As for the measurement of this part, we will choice eGFP and FCM to test the expression at 37℃. | |
===Reference=== | ===Reference=== | ||
[1] [2] Using DNA shuffling method generate thermosensitive CI repressor protein ofλphage. Shen Guo-miao.June, 2003. Dissertation Submitted to Zhejiang University For Master’s Degree. | [1] [2] Using DNA shuffling method generate thermosensitive CI repressor protein ofλphage. Shen Guo-miao.June, 2003. Dissertation Submitted to Zhejiang University For Master’s Degree. | ||
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Latest revision as of 14:55, 19 October 2016
A temperature promoter cotaining clts repressor
This is based on part(BBa_K608351).Composite promoter (BBa_K608351) include the mutant type CIλrepressor so that it can express above 37℃.Compared with the part(BBa_K608351),it is more sensitive at 37℃.According to the essay, we choose five important sites A44G, G92A, C188T, G378A, A629T, which may enhance more than tenth times expression at 37℃，compared with wild type(BBa_K608351).
Table1:Galactosidase acitivty unit after induction and compared with cIts857[1]
Table2:Nucleotide changes and the deduced amino acid changes in five thermosensitive cI repressor proteins[2]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 781 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
As for the measurement of this part, we will choice eGFP and FCM to test the expression at 37℃.
Reference
[1] [2] Using DNA shuffling method generate thermosensitive CI repressor protein ofλphage. Shen Guo-miao.June, 2003. Dissertation Submitted to Zhejiang University For Master’s Degree.