Difference between revisions of "Part:BBa K1991001"
 
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Alcohol oxidase (AOX) is an oxido-reductase that catalyzes the oxidation of alcohol and produce aldehyde and hydrogen peroxide (C<sub>2</sub>H<sub>5</sub>OH + O<sub>2</sub> → CH<sub>3</sub>CHO + H<sub>2</sub>O<sub>2</sub>). There are two Alcohol Oxidase (AOX) genes, AOX1 ([https://www.ncbi.nlm.nih.gov/protein/CAY72092.1 CAY72092.1]) and AOX2 ([https://www.ncbi.nlm.nih.gov/protein/CAY71377.1 CAY71377.1]), on chromosome 4 in  [http://bioinformatics.psb.ugent.be/genomes/view/Pichia-pastoris Pichia pastoris]. AOX was utilized to provide carbon source and energy for the methylotrophic yeast by metabolizing primary alcohol including methanol and some short-chain alcohols such as ethanol.<br />
 
Alcohol oxidase (AOX) is an oxido-reductase that catalyzes the oxidation of alcohol and produce aldehyde and hydrogen peroxide (C<sub>2</sub>H<sub>5</sub>OH + O<sub>2</sub> → CH<sub>3</sub>CHO + H<sub>2</sub>O<sub>2</sub>). There are two Alcohol Oxidase (AOX) genes, AOX1 ([https://www.ncbi.nlm.nih.gov/protein/CAY72092.1 CAY72092.1]) and AOX2 ([https://www.ncbi.nlm.nih.gov/protein/CAY71377.1 CAY71377.1]), on chromosome 4 in  [http://bioinformatics.psb.ugent.be/genomes/view/Pichia-pastoris Pichia pastoris]. AOX was utilized to provide carbon source and energy for the methylotrophic yeast by metabolizing primary alcohol including methanol and some short-chain alcohols such as ethanol.<br />
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In Mingdao iGEM 2016, the gene expression of this part was confirmed with a constitutive promoter and RBS ([https://parts.igem.org/Part:BBa_K1991003 BBa_K1991003]). In addition, the part was further applied in Blood Alcohol Meter ([https://parts.igem.org/Part:BBa_K1991009 BBa_K1991009]) by the demonstration of H2O2 enzyme activity assay and modeling on an electrochemical simulator.
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In Mingdao iGEM 2016, the gene expression of this part was confirmed with a constitutive promoter and RBS ([https://parts.igem.org/Part:BBa_K1991003 BBa_K1991003]). In addition, the part was further applied in Blood Alcohol Meter ([https://parts.igem.org/Part:BBa_K1991009 BBa_K1991009]) by the demonstration of H<sub>2</sub>O<sub>2</sub> enzyme activity assay and modeling on an electrochemical simulator.
  
 
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Latest revision as of 14:34, 19 October 2016


AOX2 (Alcohol Oxidase)

Alcohol oxidase (AOX) is an oxido-reductase that catalyzes the oxidation of alcohol and produce aldehyde and hydrogen peroxide (C2H5OH + O2 → CH3CHO + H2O2). There are two Alcohol Oxidase (AOX) genes, AOX1 (CAY72092.1) and AOX2 (CAY71377.1), on chromosome 4 in [http://bioinformatics.psb.ugent.be/genomes/view/Pichia-pastoris Pichia pastoris]. AOX was utilized to provide carbon source and energy for the methylotrophic yeast by metabolizing primary alcohol including methanol and some short-chain alcohols such as ethanol.
In Mingdao iGEM 2016, the gene expression of this part was confirmed with a constitutive promoter and RBS (BBa_K1991003). In addition, the part was further applied in Blood Alcohol Meter (BBa_K1991009) by the demonstration of H2O2 enzyme activity assay and modeling on an electrochemical simulator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]