Difference between revisions of "Part:BBa K1941006"

 
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sgRNA that targets the TDH3 promoter.
 
sgRNA that targets the TDH3 promoter.
  
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===Usage and Biology===
 
===Usage and Biology===
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The 20 bp of the TDH3 promoter targeted by this gRNA is near the transcription start site. It can therefore perform CRISPRi when complexed with dCas9.
  
 
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Revision as of 14:34, 19 October 2016


sgRNA for c6_TDH3 (1)

sgRNA that targets the TDH3 promoter.

Usage and Biology

The 20 bp of the TDH3 promoter targeted by this gRNA is near the transcription start site. It can therefore perform CRISPRi when complexed with dCas9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 145
    Illegal NgoMIV site found at 174
  • 1000
    COMPATIBLE WITH RFC[1000]