Difference between revisions of "Part:BBa K2180009"

m
Line 3: Line 3:
 
<partinfo>BBa_K2180009 short</partinfo>
 
<partinfo>BBa_K2180009 short</partinfo>
  
−
This is the assembly of our basic parts BBa_K2180004 , BBa_K2180007 and BBa_K2180008
+
Coding sequence of LacI protein + LVA (rapid degradation tail) wich improve the switch time and make it more sensitive.  
−
The NcoI restriction site is overlapping parts BBa_K2180004 and BBa_K2180007 at the Start codon of LacI + LVA.
+
−
 
+
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 14:31, 19 October 2016


LacI-LVA for B. subtilis

Coding sequence of LacI protein + LVA (rapid degradation tail) wich improve the switch time and make it more sensitive. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 178
    Illegal AgeI site found at 966
  • 1000
    COMPATIBLE WITH RFC[1000]