Difference between revisions of "Part:BBa K1896013"

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This part was used to express a monomeric Streptavidin protein ([[Part:BBa_K1896000|mSA2]]) on the cell surface of ''E. coli''. The [[Part:BBa_K1896003|INP<sub>NC</sub>]] domain, which consists of the N- and C-terminal domains of a ''Xanthomonas campestris'' ice nucleating protein, directs the fusion protein to the outer cell membrane.  
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This part was used to express a monomeric Streptavidin protein ([[Part:BBa_K1896000|mSA2]]) on the cell surface of ''E. coli''. The [[Part:BBa_K1896003|INP<sub>NC</sub>]] domain, which consists of the N- and C-terminal domains of a ''Xanthomonas campestris'' ice nucleating protein, directs the fusion protein to the outer cell membrane.[1] The UGent Belgium 2016 iGEM team made this part with the aim of attaching  ''E. coli'' cells to PLA structures coated with biotin, but could not confirm that this part improves adhesion. 
  
 
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<partinfo>BBa_K1896013 parameters</partinfo>
 
<partinfo>BBa_K1896013 parameters</partinfo>
 
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===References===
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<ol>
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<li>Wu, P. H., Giridhar, R., &amp; Wu, W. T. (2006). Surface display of transglucosidase on ''Escherichia coli'' by using the ice nucleation protein of Xanthomonas campestris and its application in glucosylation of hydroquinone. ''Biotechnology and bioengineering'', 95(6), 1138-1147.</li>
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</ol>

Latest revision as of 14:15, 19 October 2016


INP_NC-mSA2 generator

This part was used to express a monomeric Streptavidin protein (mSA2) on the cell surface of E. coli. The INPNC domain, which consists of the N- and C-terminal domains of a Xanthomonas campestris ice nucleating protein, directs the fusion protein to the outer cell membrane.[1] The UGent Belgium 2016 iGEM team made this part with the aim of attaching E. coli cells to PLA structures coated with biotin, but could not confirm that this part improves adhesion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 466
    Illegal AgeI site found at 1082
    Illegal AgeI site found at 1142
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Wu, P. H., Giridhar, R., & Wu, W. T. (2006). Surface display of transglucosidase on Escherichia coli by using the ice nucleation protein of Xanthomonas campestris and its application in glucosylation of hydroquinone. Biotechnology and bioengineering, 95(6), 1138-1147.