Difference between revisions of "Part:BBa K1896012"
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===References=== | ===References=== | ||
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| − | <li>Wu, P. H., Giridhar, R., & Wu, W. T. (2006). Surface display of transglucosidase on Escherichia coli by using the ice nucleation protein of Xanthomonas campestris and its application in glucosylation of hydroquinone. ''Biotechnology and bioengineering'', 95(6), 1138-1147.</li> | + | <li>Wu, P. H., Giridhar, R., & Wu, W. T. (2006). Surface display of transglucosidase on ''Escherichia coli'' by using the ice nucleation protein of Xanthomonas campestris and its application in glucosylation of hydroquinone. ''Biotechnology and bioengineering'', 95(6), 1138-1147.</li> |
</ol> | </ol> | ||
Latest revision as of 14:10, 19 October 2016
INP_NC-streptavidin generator
This part was designed to express the Streptavidin molecule on the cell surface of E. coli. Fusion proteins containing the INPNC domain have been described to be directed to the outer cell membrane. [1] However, it appears that the tetrameric nature of Streptavidin makes the resulting fusion protein toxic to the cell, as no correct colonies could be obtained after multiple attempts.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 466
Illegal AgeI site found at 1082
Illegal AgeI site found at 1133 - 1000COMPATIBLE WITH RFC[1000]
References
- Wu, P. H., Giridhar, R., & Wu, W. T. (2006). Surface display of transglucosidase on Escherichia coli by using the ice nucleation protein of Xanthomonas campestris and its application in glucosylation of hydroquinone. Biotechnology and bioengineering, 95(6), 1138-1147.