Difference between revisions of "Part:BBa K1896013"

Revision as of 13:57, 19 October 2016

INP_NC-mSA2 generator

This part was used to express a monomeric Streptavidin protein (mSA2) on the cell surface of E. coli. The INP_NC domain, which consists of the N- and C-terminal domains of a Xanthomonas campestris ice nucleating protein, directs the fusion protein to the outer cell membrane.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 466
Illegal AgeI site found at 1082
Illegal AgeI site found at 1142
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1896013 short</partinfo>
-This part was used to express a monomeric Streptavidin protein ([[Part:BBa_K1896000|mSA2]]) on the cell surface of ''E. coli''. The the [[Part:BBa_K1896003|INP<sub>NC</sub>]] domain, which consists of the N- and C-terminal domains of a ''Xanthomonas campestris'' ice nucleating protein, directs the fusion protein to the outer cell membrane.
+This part was used to express a monomeric Streptavidin protein ([[Part:BBa_K1896000|mSA2]]) on the cell surface of ''E. coli''. The [[Part:BBa_K1896003|INP<sub>NC</sub>]] domain, which consists of the N- and C-terminal domains of a ''Xanthomonas campestris'' ice nucleating protein, directs the fusion protein to the outer cell membrane.
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