Difference between revisions of "Part:BBa K2170001:Design"

Revision as of 12:59, 19 October 2016

Secretory eukaryotic biotin binding receptor with enhanced monomeric avidin

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 576
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2432

Design Notes

Related BioBrick:
Other versions:BBa_K2170002: Secretory eukaryotic biotin binding receptor with single chain avidin
Related BioBricks:BBa_K2170003: Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA

Cloning details:
Designed in RFC25 Mutation C889G to delete XbaI restriction site Truncation upstream/downstream compared to template, ?explanation?

Quality control measures:
Test digestion using EcorRI & PstI Sequencing using VF2 Part was partly sequenced

Backbone:
Backbone name: pSB1C3 Resistance: Cp

Corresponding part author/authors:

Source

Source:
Parts synthesized by IDT

Organism:
Genesequence derived from Escherichia coli

References

Literature references:

Database references:

@@ Line 9: / Line 9: @@
 '''Related BioBrick:'''<br>
-Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170002 BBa_K2170002: Secretory eukaryotic biotin binding receptor with single chain avidin]<br>
+Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170002 BBa_K2170002]: Secretory eukaryotic biotin binding receptor with single chain avidin <br>
-Related BioBricks:[https://parts.igem.org/wiki/index.php?title=BBa_K2170003 BBa_K2170003: Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA]
+Related BioBricks:[https://parts.igem.org/wiki/index.php?title=BBa_K2170003 BBa_K2170003]: Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA
 '''Cloning details:'''<br>