Difference between revisions of "Part:BBa K2100008:Experience"
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===Applications of BBa_K2100008=== | ===Applications of BBa_K2100008=== | ||
| + | This entry vector was recombined into an expression vector pPRE3:eYFP with an LR reaction. The construct was characterized in tHESC cells. | ||
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| + | We transfection pPRE3:eYFP into tHESC to test its on/off functionality by comparing cells induced with MPA and cells induced with 1 uM MPA. We transfected hEF1a:mKate into tHESC as well as a transfection marker. | ||
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| + | [image here] | ||
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| + | The results showed little to no increase in fold difference of yellow fluorescence when comparing induced vs. uninduced cells. Unfortunately, we could not prove with this experiment that the PRE3 promoter increases activity in response to progesterone induction. | ||
| + | |||
| + | Note: We attempted to characterize pPRE3 in MCF-7 cells as well, however due to low transfection efficiency we were unable to get conclusive results about the on-off functionality of the promoter. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 12:56, 19 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2100008
This entry vector was recombined into an expression vector pPRE3:eYFP with an LR reaction. The construct was characterized in tHESC cells.
We transfection pPRE3:eYFP into tHESC to test its on/off functionality by comparing cells induced with MPA and cells induced with 1 uM MPA. We transfected hEF1a:mKate into tHESC as well as a transfection marker.
[image here]
The results showed little to no increase in fold difference of yellow fluorescence when comparing induced vs. uninduced cells. Unfortunately, we could not prove with this experiment that the PRE3 promoter increases activity in response to progesterone induction.
Note: We attempted to characterize pPRE3 in MCF-7 cells as well, however due to low transfection efficiency we were unable to get conclusive results about the on-off functionality of the promoter.
User Reviews
UNIQ737eb77a784d2489-partinfo-00000000-QINU UNIQ737eb77a784d2489-partinfo-00000001-QINU