Difference between revisions of "Part:BBa K2170001:Design"
Line 9: Line 9:
  
 
'''Related BioBrick:'''
 
'''Related BioBrick:'''
−
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
+
Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170002 BBa_K2170002: Secretory eukaryotic biotin binding receptor with single chain avidin]
−
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
+
Related BioBricks:[https://parts.igem.org/wiki/index.php?title=BBa_K2170003 BBa_K2170003: Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA]
  
 
'''Cloning details:'''<br>
 
'''Cloning details:'''<br>
−
<!--*Designed in RFC10/RFC23/RFC25-->
+
Designed in RFC25
−
<!--*Mutation C889G to delete XbaI restriction site-->
+
Mutation C889G to delete XbaI restriction site
−
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
+
Truncation upstream/downstream compared to template, ?explanation?
  
 
'''Quality control measures:'''<br>
 
'''Quality control measures:'''<br>
−
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
+
Test digestion using EcorRI & PstI
−
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
+
Sequencing using VF2
−
<!--*Part was partly sequenced/Part was totally sequenced-->
+
Part was partly sequenced
  
 
'''Backbone:'''<br>
 
'''Backbone:'''<br>
−
<!--*Backbone name: pSB1C3'/?backbone_name?-->
+
Backbone name: pSB1C3
−
<!--*Resistance: Amp/Cp/Kan/-->
+
Resistance: Cp
−
<!--*Copynumber: low/medium/high-->
+
<!--*Copynumber: high
  
 
'''Protein coding:'''<br>
 
'''Protein coding:'''<br>
−
<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
+
Protein: Secretory eukaryotic biotin binding receptor with single chain avidin [Nucleotide 633  to 3314]
−
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
+
 
−
<!--*The protein encoded is posttranslationally modified by ???.-->
+
 
−
<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
+
  
 
'''Enzymatic activity:'''
 
'''Enzymatic activity:'''
−
<!--none/EC-number ?.?.?.?-->
+
none
  
 
'''Cytotoxicity:'''<br>
 
'''Cytotoxicity:'''<br>
−
<!--none/not known/cytotoxic for ''organism name''-->
+
not known
  
 
'''Safety notes:'''<br>
 
'''Safety notes:'''<br>
−
<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
+
Known and anticipated sefety issues: none
−
<!--Known and anticipated security issues: none/other.-->
+
<!--Known and anticipated security issues: none
  
 
'''Intellectual property:'''
 
'''Intellectual property:'''
Line 57: Line 56:
 
<!--*Preexisting BioBrick ?Bba_number?-->
 
<!--*Preexisting BioBrick ?Bba_number?-->
 
<!--*cDNA Clone: ?clone_name?, ?company_name?-->
 
<!--*cDNA Clone: ?clone_name?, ?company_name?-->
−
<!--*Synthesized by ?company_name?.-->
+
Parts synthesized by IDT
  
 
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
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'''Literature references:'''<br>
 
'''Literature references:'''<br>
−
<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
+
<!--*[http://www.nature.com/nmeth/journal/v2/n2/full/nmeth735.html >Chen, I., Howarth, M., Lin, W., & Ting, A. Y. (2005). Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase. Nature methods, 2(2), 99-104.]-->
  
 
'''Database references:'''<br>
 
'''Database references:'''<br>

Revision as of 12:55, 19 October 2016


Secretory eukaryotic biotin binding receptor with enhanced monomeric avidin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2432


Design Notes

Related BioBrick: Other versions:BBa_K2170002: Secretory eukaryotic biotin binding receptor with single chain avidin Related BioBricks:BBa_K2170003: Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA

Cloning details:
Designed in RFC25 Mutation C889G to delete XbaI restriction site Truncation upstream/downstream compared to template, ?explanation?

Quality control measures:
Test digestion using EcorRI & PstI Sequencing using VF2 Part was partly sequenced

Backbone:
Backbone name: pSB1C3 Resistance: Cp

Corresponding part author/authors:

Source

Source:
Parts synthesized by IDT


Organism:
Genesequence derived from Escherichia coli


References

Literature references:

Database references: