Difference between revisions of "Part:BBa K1930005:Design"
 
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===Design Notes===
 
===Design Notes===
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The promoter P<sub>AtpI</sub> is a constitutive promoter which has its origin in <em>Bacillus subtilis</em>. It is responsible for the expression of <em>atpA</em> gene (ATP synthesis) during the first 30 minutes of the germination of <em>B. subtilis</em>. <em>atpA</em> gene is part of an operon (<em>atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC</em>), therefore the promoter region in front of the first protein coding gene (<em>atpI</em>) in this operon was chosen.
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<p> The promoter P<sub>AtpI</sub> is a constitutive promoter which has its origin in <em>Bacillus subtilis</em>. It is responsible for the expression of <em>atpA</em> gene (ATP synthesis) during the first 30 minutes of the germination of <em>B. subtilis</em>. <em>atpA</em> gene is part of an operon (<em>atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC</em>), therefore the promoter region in front of the first protein coding gene (<em>atpI</em>) in this operon was chosen.</p>
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For more information see plasmid construction under our lab journal: http://2016.igem.org/Team:Groningen/Experiments
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<p>For more information see plasmid construction under our lab journal: (<a href="http://2016.igem.org/Team:Groningen/Labjournal#PatpI-in-pSB1C3">BBa_K1930005</a>.)</p>
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===Source===
 
===Source===
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===References===
 
===References===
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<p>[https://www.ncbi.nlm.nih.gov/pubmed/25661487 ''Sinai et al. 2015''] </p>
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<p> <a href="https://www.ncbi.nlm.nih.gov/pubmed/25661487">Sinai et al. 2015</a> </p>
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<p>[http://subtiwiki.uni-goettingen.de/bank/index.php?gene=atpA Subtiwiki-atpA]</p>
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<p><a href="http://subtiwiki.uni-goettingen.de/bank/index.php?gene=atpA">Subtiwiki-atpA</a></p>

Latest revision as of 12:47, 19 October 2016


PAtpI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter PAtpI is a constitutive promoter which has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 minutes of the germination of B. subtilis. atpA gene is part of an operon (atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC), therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen.

For more information see plasmid construction under our lab journal: (BBa_K1930005.)

Source

Ordered as gBlock. IDT (Integrated DNA technologies).

References

Sinai et al. 2015

Subtiwiki-atpA