Difference between revisions of "Part:BBa K1919214"
 
Line 3: Line 3:
 
<partinfo>BBa_K1919214 short</partinfo>
 
<partinfo>BBa_K1919214 short</partinfo>
  
−
This device can express AarC with His-tag. Promoter is BBa_J23114. His-tag and T7 terminator come from pET-23 vector.
+
This device can express AarC with His-tag. Promoter is BBa_J23114. His-tag and T7 terminator come from pET-23 vector. About AarC, please see <partinfo>BBa_K1919233</partinfo> (AarC coding sequence) page.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 19: Line 19:
 
<partinfo>BBa_K1919214 parameters</partinfo>
 
<partinfo>BBa_K1919214 parameters</partinfo>
 
<!-- -->
 
<!-- -->
−
 
−
[[File:EH-0.png|450px|thumb|left|'''Figure 1:''' Fermentation of ''E.&nbsp;coli'' KRXwith <partinfo>BBa_K863005</partinfo> (ECOL) in an Infors Labfors Bioreactor, scale: 3&nbsp;L, [http://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium] + 60&nbsp;µg/mL chloramphenicol, 37&nbsp;°C, pH&nbsp;7, agitation on cascade to hold pO<sub>2</sub> at 50&nbsp;%, OD<sub>600</sub> measured every 30&nbsp;minutes.]]
 

Latest revision as of 12:18, 19 October 2016


J23114 promoter-Constitutive AarC Expression Device

This device can express AarC with His-tag. Promoter is BBa_J23114. His-tag and T7 terminator come from pET-23 vector. About AarC, please see BBa_K1919233 (AarC coding sequence) page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 168
    Illegal AgeI site found at 264
    Illegal AgeI site found at 276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 114
    Illegal BsaI.rc site found at 378