Difference between revisions of "Part:BBa K1919200"

Revision as of 12:01, 19 October 2016

J23100 promoter-Constitutive AarC Expression Device

This device can express AarC with His-tag. Promoter is BBa_J23100. His-tag and T7 terminator come from pET-23 vector.

AarC can let

Figure 1: ABC

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1578
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 168
Illegal AgeI site found at 264
Illegal AgeI site found at 276
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 114
Illegal BsaI.rc site found at 378

Revision as of 11:58, 19 October 2016 (view source) Steam (Talk \| contribs) (→‎Usage and Biology) ← Older edit		Revision as of 12:01, 19 October 2016 (view source) Steam (Talk \| contribs) (→‎Usage and Biology) Newer edit →
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	AarC can let		AarC can let

−	[[File:EH-K.png\|~~800px~~\|thumb\|left\|'''Figure 1:''' Fermentation of ''E. coli'' KRXwith <partinfo>BBa_K863005</partinfo> (ECOL) in an Infors Labfors Bioreactor, scale: 3 L, [http://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium] + 60 µg/mL chloramphenicol, 37 °C, pH 7, agitation on cascade to hold pO<sub>2</sub> at 50 %, OD<sub>600</sub> measured every 30 minutes.]]	+	[[File:EH-K.png\|1000px\|thumb\|left\|'''Figure 1:''' ABC]]