Difference between revisions of "Part:BBa K1913016"

Revision as of 11:45, 19 October 2016

434- and lambda cI balance RFP reporter

434- and lambda cI balance RFP reporter

Usage and Biology

We have combined BBa_K1913016 and BBa_K1913007 into BBa_K1913006, inserted an RBS library (see design notes) upstream of the 434 cI gene and transformed it to DH5alpha. DH5α cells transformed with the BBa_K1913006 plasmid yielded clones with varying intensities of red coloring on agar (without both L-Arabinose and D-Glucose) plates (Figure 1). The variation in the red intensity is seen very clearly between the top three circled colonies in Figure 12.This diversity is an indication that BBa_K1913016 works as intended: variation in the 434 cI RBS should cause different ratios between λ and 434 cI protein levels, leading to differences in mRFP expression.

Figure 11. Close-up picture of culture plate with colonies containing the subpopulation two-operon plasmid (including the RBS library). The blue circled and (faintly) red circled colonies were selected to be used in separate plate reader experiments.

Sequence and Features