Difference between revisions of "Part:BBa K1919200"
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This device can express AarC with His-tag. Promoter is BBa_J23100. His-tag and T7 terminator come from pET-23 vector. | This device can express AarC with His-tag. Promoter is BBa_J23100. His-tag and T7 terminator come from pET-23 vector. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | [[File:EH-K.png|800px|thumb|left|'''Figure 1:''' Fermentation of ''E. coli'' KRXwith <partinfo>BBa_K863005</partinfo> (ECOL) in an Infors Labfors Bioreactor, scale: 3 L, [http://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium] + 60 µg/mL chloramphenicol, 37 °C, pH 7, agitation on cascade to hold pO<sub>2</sub> at 50 %, OD<sub>600</sub> measured every 30 minutes.]] | |
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Revision as of 11:38, 19 October 2016
J23100 promoter-Constitutive AarC Expression Device
This device can express AarC with His-tag. Promoter is BBa_J23100. His-tag and T7 terminator come from pET-23 vector.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1578
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 168
Illegal AgeI site found at 264
Illegal AgeI site found at 276 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 114
Illegal BsaI.rc site found at 378