Difference between revisions of "Part:BBa K1941004"

 
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<partinfo>BBa_K1941004 short</partinfo>
 
<partinfo>BBa_K1941004 short</partinfo>
  
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Scaffold RNA that targets the region c6 of CYC1 promoter and recruits two times the protein repressor module PCP-Mxi via two effector protein recruitments site PP7. PP7 is a well-characterized viral RNA sequence which is recognized by PCP protein. This RNA is composed of the dCas9-binding sgRNA that interacts with CYC1, and two times the PCP-binding RNA hairpin, PP7.
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Scaffold RNA with PP7 hairpin that targets the region c6 of CYC1 promoter
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===Usage and Biology===
 
===Usage and Biology===
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A scaffold RNA is a guide RNA (gRNA), supplemented with one or more additional hairpins that can recruit different RNA binding modules. In this case, the hairpins are two times PP7, PP7 being a well-characterized viral RNA sequence which is recognized by PCP RNA binding module. This allows the recruitment of fusion proteins, like BBa_K1941000, to act on the level of expression of the promoter CYC1.
  
 
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Revision as of 11:33, 19 October 2016


scCyc1_2PP7

Scaffold RNA with PP7 hairpin that targets the region c6 of CYC1 promoter


Usage and Biology

A scaffold RNA is a guide RNA (gRNA), supplemented with one or more additional hairpins that can recruit different RNA binding modules. In this case, the hairpins are two times PP7, PP7 being a well-characterized viral RNA sequence which is recognized by PCP RNA binding module. This allows the recruitment of fusion proteins, like BBa_K1941000, to act on the level of expression of the promoter CYC1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 231
    Illegal NgoMIV site found at 260
  • 1000
    COMPATIBLE WITH RFC[1000]