Difference between revisions of "Part:BBa K2066548"
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<partinfo>BBa_K2066548 short</partinfo> | <partinfo>BBa_K2066548 short</partinfo> | ||
| − | + | Sequence from Amit, R., Garcia, H. G., Phillips, R. & Fraser, S. E. Building enhancers from the ground up: a synthetic biology approach. Cell146, 105–118 (2011). Binding cassette that is cloned in the spacer region between the enhancer and promoter and holds two TetO (TetR respressor) binding sites. IN this case, the transfer function should theoretically have three discrete states (unrepressed, intermediate, fully repressed). | |
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Latest revision as of 11:15, 19 October 2016
Synthetic Enhancer Project: 57S (2 Tet binding cassette)
Sequence from Amit, R., Garcia, H. G., Phillips, R. & Fraser, S. E. Building enhancers from the ground up: a synthetic biology approach. Cell146, 105–118 (2011). Binding cassette that is cloned in the spacer region between the enhancer and promoter and holds two TetO (TetR respressor) binding sites. IN this case, the transfer function should theoretically have three discrete states (unrepressed, intermediate, fully repressed).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 24
Illegal NheI site found at 119 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 64
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]