Difference between revisions of "Part:BBa K2066546"

 
 
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<partinfo>BBa_K2066546 short</partinfo>
 
<partinfo>BBa_K2066546 short</partinfo>
  
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tempt
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Sequence from Amit, R., Garcia, H. G., Phillips, R. & Fraser, S. E. Building enhancers from the ground up: a synthetic biology approach. Cell146, 105–118 (2011).
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NRI coding region.  NRI will get phosophorylated by NRII2302 kinase and this activated version can bind to the enhancer and when the DNA forms a loop, it can bind to the promoter poised with a sigma 54 RNA poly and initiate transcription.
  
 
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Latest revision as of 11:09, 19 October 2016


Synthetic Enhancer Project: glnG (NRI)

Sequence from Amit, R., Garcia, H. G., Phillips, R. & Fraser, S. E. Building enhancers from the ground up: a synthetic biology approach. Cell146, 105–118 (2011).

NRI coding region. NRI will get phosophorylated by NRII2302 kinase and this activated version can bind to the enhancer and when the DNA forms a loop, it can bind to the promoter poised with a sigma 54 RNA poly and initiate transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 519
  • 1000
    COMPATIBLE WITH RFC[1000]