Difference between revisions of "Part:BBa K2066545"
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<partinfo>BBa_K2066545 short</partinfo> | <partinfo>BBa_K2066545 short</partinfo> | ||
| − | + | Sequence from Amit, R., Garcia, H. G., Phillips, R. & Fraser, S. E. Building enhancers from the ground up: a synthetic biology approach. Cell146, 105–118 (2011). LacO sequence included in the circuit so that LacI can bind and inhibit initial transcription of the NRI and reporter gene. Later, saturating levels of IPTG is added to block the LacI binding and repression so that the circuit could work. | |
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Latest revision as of 11:00, 19 October 2016
Synthetic Enhancer Project: LacI binding site (LacO)
Sequence from Amit, R., Garcia, H. G., Phillips, R. & Fraser, S. E. Building enhancers from the ground up: a synthetic biology approach. Cell146, 105–118 (2011). LacO sequence included in the circuit so that LacI can bind and inhibit initial transcription of the NRI and reporter gene. Later, saturating levels of IPTG is added to block the LacI binding and repression so that the circuit could work.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]