Difference between revisions of "Part:BBa K1913007"

Revision as of 10:45, 19 October 2016

434- and lambda cI operon for tuning protein balance

This part is intended to provide a balance between the 434- and lambda cI proteins depending on the strength of the RBS that is inserted upstream of 434 cI.

Usage and Biology

Before using this part, read the design notes. This part contains two viral genes: 434- and lambda cI (BBa_C0052 and part of BBa_K081007) expressed under the pBAD/AraC promoter (BBa_I0500). The 434 cI gene is not yet preceded by an RBS, allowing tuning of the cI protein balance. The 434 cI repressor protein encoded in this part should be more rapidly degraded (see design considerations) than the lambda cI protein. Therefore, changing the strength of the pBAD promoter in this part (with L-Arabinose or D-Glucose) should temporary shift the ratio between the two cI protein levels. We recommend using this part together with the modified lambda Prm promoter (BBa_I12006). Combining these parts should lead to interesting dynamics of expression under this modified lambda Prm promoter in response to changes in pBAD promoter strength.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1898
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 8: / Line 8: @@
 ===Usage and Biology===
-<b>Before using this part, read the design notes.</b>This part contains two viral genes: 434- and lambda cI (BBa_C0052 and part of BBa_K081007) expressed under the pBAD/AraC promoter (BBa_I0500). The 434 cI gene is not yet preceded by an RBS, allowing tuning of the cI protein balance. The 434 cI repressor protein encoded in this part should be more rapidly degraded (see design considerations) than the lambda cI protein.
+<b>Before using this part, read the design notes.</b> This part contains two viral genes: 434- and lambda cI (BBa_C0052 and part of BBa_K081007) expressed under the pBAD/AraC promoter (BBa_I0500). The 434 cI gene is not yet preceded by an RBS, allowing tuning of the cI protein balance. The 434 cI repressor protein encoded in this part should be more rapidly degraded (see design considerations) than the lambda cI protein.
 Therefore, changing the strength of the pBAD promoter in this part (with L-Arabinose or D-Glucose) should temporary shift the ratio between the two cI protein levels. We recommend using this part together with the modified lambda Prm promoter (BBa_I12006). Combining these parts should lead to interesting dynamics of expression under this modified lambda Prm promoter in response to changes in pBAD promoter strength.