Difference between revisions of "Part:BBa K2040121:Design"

(→‎Design Notes)
(→‎Design Notes)
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Two illegal cutsites (EcoRI and XbaI) were subsequently removed via site-directed mutagenesis.
 
Two illegal cutsites (EcoRI and XbaI) were subsequently removed via site-directed mutagenesis.
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 +
This device doesn't include a RBS because it's for fungal expression.
  
 
===Source===
 
===Source===

Revision as of 09:16, 19 October 2016


PgpdA + KillerRed + TtrpC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 880
    Illegal BsaI.rc site found at 1171


Design Notes

KillerRed can be replaced by KillerRed-NLS so that KillerRed can be localized in the nucleus to increase efficiency of KillerRed-mediated oxidative stress.

Two illegal cutsites (EcoRI and XbaI) were subsequently removed via site-directed mutagenesis.

This device doesn't include a RBS because it's for fungal expression.

Source

KillerRed was engineered from anm2CP.1[1]

PgpdA,TtrpC ,from Aspergillus nidulans, were isolated from vector pBARGPE1.

References

[1]Bulina, Maria E, Chudakov, Dmitriy M, Britanova, Olga V, Yanushevich, Yurii G, Staroverov, Dmitry B, Chepurnykh, Tatyana V, Merzlyak, Ekaterina M, Shkrob, Maria A, Lukyanov, Sergey, Lukyanov and Konstantin A.
A Genetically Encoded Photosensitizer
Nature Biotechnology 2006 24: 95-99. http://dx.doi.org/10.1038/nbt1175