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Revision as of 09:08, 19 October 2016


J23100 promoter-Constitutive AarC Expression Device

This device can express AarC with His-tag. Promoter is BBa_J23100. His-tag and T7 terminator come from pET-23 vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 168
    Illegal AgeI site found at 264
    Illegal AgeI site found at 276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 114
    Illegal BsaI.rc site found at 378



EH-0.png