Difference between revisions of "Part:BBa K1926003:Design"
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| + | Details are all at the [https://parts.igem.org/Part:BBa_K1926001 main page]. | ||
===Source=== | ===Source=== | ||
Latest revision as of 07:17, 19 October 2016
A cyclic promoter of CDK4 from human genome
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 522
Illegal NheI site found at 526 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 531
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 142
Illegal AgeI site found at 173 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We got the sequence from human genome through PCR using the following primers:
pCDK4-F: TGGTGGAGCGAAAAGGTGACAGCATC
pCDK4-R: GCGGAAACTGGGAGGGCGGGGCGAA
(Product: 457)
The point mutation C→A was done at 284 to remove PstⅠ restriction site.
Details are all at the main page.
Source
The sequence was retrieved from Addgene.
References
1. Guo, Z.Y., et al., The elements of human cyclin D1 promoter and regulation involved. Clin Epigenetics, 2011. 2(2): p. 63-76.