Difference between revisions of "Part:BBa K2172001:Design"
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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Two extra sticky ends are added to the gene when it is cloned via PCR. They are needed so that the part can be loaded onto other vectors.
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Two extra sticky ends are added to the gene when it is cloned via PCR. They are needed so that the part can be loaded onto other vectors. Site-directed mutagenesis in SmCPS1 is performed to alter the triplets identical to the sequence of restriction enzymes used to remove the part from the vector.
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SmCPS1 is long and complicated. The original SmCPS1 has many restriction enzymes binding sites on it, and could not be loaded onto iGEM competition vectors. Therefore, site-directed mutagenesis must be used on the gene to remove the binding sites.
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===Source===
 
===Source===

Latest revision as of 07:10, 19 October 2016


-SmCPS1-TEV-GFP-


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1896
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 657
    Illegal BamHI site found at 255
    Illegal XhoI site found at 391
    Illegal XhoI site found at 415
    Illegal XhoI site found at 1708
    Illegal XhoI site found at 1918
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 60
    Illegal NgoMIV site found at 1477
    Illegal NgoMIV site found at 2041
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Two extra sticky ends are added to the gene when it is cloned via PCR. They are needed so that the part can be loaded onto other vectors. Site-directed mutagenesis in SmCPS1 is performed to alter the triplets identical to the sequence of restriction enzymes used to remove the part from the vector.

Source

This gene comes from the genome of Salvia miltiorrhiza.

References