Difference between revisions of "Part:BBa K2172006:Design"
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===Source=== | ===Source=== | ||
− | The source of the part is the | + | The source of the part is the plant Salvia miltiorrhiza. |
===References=== | ===References=== |
Revision as of 06:57, 19 October 2016
-SmCPS1-
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1896
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 657
Illegal BamHI site found at 255
Illegal XhoI site found at 391
Illegal XhoI site found at 415
Illegal XhoI site found at 1708
Illegal XhoI site found at 1918 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 60
Illegal NgoMIV site found at 1477
Illegal NgoMIV site found at 2041 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Two extra sticky ends are added to the gene when it is cloned via PCR. They are needed so that the part can be loaded onto other vectors. Site-directed mutagenesis is performed to alter the triplets identical to the sequence of restriction enzymes used to remove the part from the vector.
Source
The source of the part is the plant Salvia miltiorrhiza.