Difference between revisions of "Part:BBa K1926001:Design"

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(Product: 986)
 
(Product: 986)
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Details are all at the [https://parts.igem.org/Part:BBa_K1926001 main page].
  
 
===Source===
 
===Source===

Revision as of 06:36, 19 October 2016


A cyclic promoter of Cyclin E from human genome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 382
    Illegal NotI site found at 497
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 529
    Illegal XhoI site found at 1050
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 273
    Illegal NgoMIV site found at 313
    Illegal NgoMIV site found at 491
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We got this sequence from human genome through PCR using the following primers:

pCCNE-F: CGTGTTTACATTCCACCCGCGCCA

pCCNE-R: TGATGGGGCTGCTCCGGCCT

(Product: 986)

Details are all at the main page.

Source

The sequence was retrieved from Addgene.

References

1. Hwang, H.C. and B.E. Clurman, Cyclin E in normal and neoplastic cell cycles. Oncogene, 2005. 24(17): p. 2776-86.

2. Ohtani, K., J. DeGregori, and J.R. Nevins, Regulation of the cyclin E gene by transcription factor E2F1. Proc Natl Acad Sci U S A, 1995. 92(26): p. 12146-50.