Difference between revisions of "Part:BBa K1161003"
(Added reference to codon-optimized part as contribution) |
|||
| Line 5: | Line 5: | ||
Violacein C (Vio)C is the first of 5 protein coding sequences contained in the Violacein operon originally derived from the organism, Chromobacterium violaceum. The entire operon was submitted to the registry by the Cambridge team in 2009 as K274002. Together these five proteins form a metabolic pathway that produces the violet pigment violacein when expressed in E. coli. Like its other four partners we have appended the coding sequence with the ribosome binding site BBa_B003 so that operons that vary in gene order can be created by standard assembly means. Here we use these parts to show gene order affects pigment colour. | Violacein C (Vio)C is the first of 5 protein coding sequences contained in the Violacein operon originally derived from the organism, Chromobacterium violaceum. The entire operon was submitted to the registry by the Cambridge team in 2009 as K274002. Together these five proteins form a metabolic pathway that produces the violet pigment violacein when expressed in E. coli. Like its other four partners we have appended the coding sequence with the ribosome binding site BBa_B003 so that operons that vary in gene order can be created by standard assembly means. Here we use these parts to show gene order affects pigment colour. | ||
| − | + | ===Part Contributions=== | |
| − | + | Team Washington 2016 has codon optimized this part for use in S. cerevisiae as an improvement to its functionality in that organism. The new part, along with its further documentation, can be found at https://parts.igem.org/Part:BBa_K2165000 | |
| − | as part K2165000 | + | as part K2165000. |
| − | + | ||
| − | + | ||
| − | + | ||
Latest revision as of 06:14, 19 October 2016
Violacein C
Violacein C (Vio)C is the first of 5 protein coding sequences contained in the Violacein operon originally derived from the organism, Chromobacterium violaceum. The entire operon was submitted to the registry by the Cambridge team in 2009 as K274002. Together these five proteins form a metabolic pathway that produces the violet pigment violacein when expressed in E. coli. Like its other four partners we have appended the coding sequence with the ribosome binding site BBa_B003 so that operons that vary in gene order can be created by standard assembly means. Here we use these parts to show gene order affects pigment colour.
Part Contributions
Team Washington 2016 has codon optimized this part for use in S. cerevisiae as an improvement to its functionality in that organism. The new part, along with its further documentation, can be found at https://parts.igem.org/Part:BBa_K2165000 as part K2165000.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1312
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]