Difference between revisions of "Part:BBa K2100038"

 
 
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<partinfo>BBa_K2100038 short</partinfo>
 
<partinfo>BBa_K2100038 short</partinfo>
  
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This part is the result of a gateway combining a tet response element promoter (TRE) and the repressor TALER21 (Tal21). The repressor can bind to sites on the Tal21 promoter.
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This construct is an expression vector containing the TRE promoter upstream of the TALER21 gene. The promoter is structured as six tetO sites upstream of a minimal CMV promoter. The induction of doxycycline activates a tetracycline transactivator protein which in turn binds to the tetO sites to initiate transcription of the TALER21 repressor. TALER14 is an engineered repressor from TAL effectors, amino acids which have corresponding DNA target sites. When transcribed, TALER21 represses the DNA sequences found on the pTALER21 promoter with the help of the corepressor VP16-Gal4.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 06:00, 19 October 2016


pEXPR TRE - TALER21

This construct is an expression vector containing the TRE promoter upstream of the TALER21 gene. The promoter is structured as six tetO sites upstream of a minimal CMV promoter. The induction of doxycycline activates a tetracycline transactivator protein which in turn binds to the tetO sites to initiate transcription of the TALER21 repressor. TALER14 is an engineered repressor from TAL effectors, amino acids which have corresponding DNA target sites. When transcribed, TALER21 represses the DNA sequences found on the pTALER21 promoter with the help of the corepressor VP16-Gal4.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 344
    Illegal EcoRI site found at 520
    Illegal XbaI site found at 30
    Illegal XbaI site found at 495
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 344
    Illegal EcoRI site found at 520
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 344
    Illegal EcoRI site found at 520
    Illegal BamHI site found at 649
    Illegal XhoI site found at 586
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 344
    Illegal EcoRI site found at 520
    Illegal XbaI site found at 30
    Illegal XbaI site found at 495
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 344
    Illegal EcoRI site found at 520
    Illegal XbaI site found at 30
    Illegal XbaI site found at 495
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 664